Image Analysis Project
🧠Image Analysis Project: Brain Cell Counting
Brain cell counting using CellProfiler.
In this project, we used CellProfiler to quantify brain cells from microscopy images. This approach allowed high-throughput, automated, and reproducible cell counting.
✅ Key Analysis Steps in CellProfiler:
- Image Preprocessing using ColorToGray module.
- Applied filters:
- EnhanceEdges
- GaussianFilter
- ErodeImage
- Closing
- Identified primary objects (brain cells) for quantification.
- Exported data for statistical analysis.
📊 Example Results:
- Total cells detected: 1,245 cells per image (average).
- Increased cell density observed in specific brain regions under experimental conditions.
You can learn more about CellProfiler here.