🧠 Image Analysis Project: Brain Cell Counting

Image Analysis Project

Brain cell counting using CellProfiler.

View Project


In this project, we used CellProfiler to quantify brain cells from microscopy images. This approach allowed high-throughput, automated, and reproducible cell counting.

✅ Key Analysis Steps in CellProfiler:

  • Image Preprocessing using ColorToGray module.
  • Applied filters:
    • EnhanceEdges
    • GaussianFilter
    • ErodeImage
    • Closing
  • Identified primary objects (brain cells) for quantification.
  • Exported data for statistical analysis.

📊 Example Results:

  • Total cells detected: 1,245 cells per image (average).
  • Increased cell density observed in specific brain regions under experimental conditions.

You can learn more about CellProfiler here.